研究报告/Research Report

甜瓜CmERFIV-3基因cDNA的克隆及功能初步分析  

刘畅 , 灵芝 , 张鸿 , 郝金凤 , 哈斯阿古拉*
内蒙古大学生命科学学院, 内蒙古自治区牧草与特色作物生物技术重点实验室, 呼和浩特, 010021
作者    通讯作者
《分子植物育种》印刷版, 2018 年, 第 16卷, 第 11 篇   
收稿日期: 2017年06月01日    接受日期: 2017年06月23日    发表日期: 2018年03月10日
© 2018 BioPublisher 生命科学中文期刊出版平台
这是一篇采用Creative Commons Attribution License进行授权的开放取阅论文。只要对本原作有恰当的引用,版权所有人允许和同意第三方无条件的使用与传播。
摘 要

为了研究甜瓜ERF (ethylene-responsive factor)亚家族基因在果实成熟过程中的功能,本研究以甜瓜(Cucumis melo L.)品种河套蜜瓜为材料,克隆得到甜瓜ERF亚家组成员之一CmERFIV-3 (MELO3C021306)基因cDNA,其开放阅读框(ORF)长为702 bp,编码233个氨基酸。分别构建了CmERFIV-3基因的超表达载体pPZPIV-3和RNAi载体pARTIV-3,采用农杆菌介导的瞬时表达技术将含有重组质粒的农杆菌菌液注射到成熟期甜瓜果实中。结果显示,注射超表达载体的部位与对照相比,23.3%样品出现提前变黄的表型,而注射RNAi载体的部位与对照相比,20%样品出现持绿的表型。实时荧光定量PCR结果表明,注射超表达载体部位的目的基因的表达量是对照的2.37倍,而注射RNAi载体部位的目的基因的表达量是对照的0.24倍。这些结果初步表明CmERFIV-3基因参与甜瓜果实成熟过程。

关键词
甜瓜;ERF基因;转录因子;果实;瞬时表达
《分子植物育种》印刷版
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